Molecular Cloning of a Vaccine Antigen against Infection with the Larval Stage of Echinococcus multilocularis
نویسندگان
چکیده
منابع مشابه
Molecular cloning of a vaccine antigen against infection with the larval stage of Echinococcus multilocularis.
Alveolar and cystic hydatidosis are caused by infection with the larval stages of Echinococcus multilocularis and Echinococcus granulosus, respectively. A host-protective antigen has been identified in E. granulosus. Here we identify the presence of a closely related protein in E. multilocularis, characterize and express a cDNA encoding the antigen (designated EM95), determine the structure of ...
متن کاملIsolation and characterization of a diagnostic polysaccharide antigen from larval Echinococcus multilocularis.
A diagnostic antigen, C-antigen, was evaluated in serodiagnosis of alveolar hydatid disease by Western blotting. The C-antigen migrated diffusely to the region of 30-35 kDa in 8% polyacrylamide-SDS gel electrophoresis. We isolated this antigen from a crude metacestode extract by a simple method of phenol/chloroform extraction. The isolated antigen was resistant to boiling, proteolysis and acid ...
متن کاملMolecular cloning, expression, and serological evaluation of an 8-kilodalton subunit of antigen B from Echinococcus multilocularis.
Full-length cDNA and genomic DNA encoding an 8-kDa subunit of antigen B from Echinococcus multilocularis (designated EmAgB8/1) were isolated from an E. multilocularis metacestode cDNA library and a protoscolex genomic DNA library, respectively. The open reading frame of the cDNA clone encodes a polypeptide comprising 85 amino acids with a 20-amino-acid NH(2)-terminal signal sequence, which was ...
متن کاملAnthelmintic Baiting of Foxes against Urban Contamination with Echinococcus multilocularis
recent years, increases in the urban fox population have been observed in many countries of the Northern Hemisphere. As a result, Echinococcus multilocularis has entered the urban environment. Because of a possible increased risk for alveolar echinococcosis, intervention strategies need to be evaluated. In Zürich, Switzerland, 50 praziquantel-containing baits per km 2 were distributed monthly i...
متن کاملNatural larval Echinococcus multilocularis infection in a Norway rat, Rattus norvegicus, captured indoors in Hokkaido, Japan
Natural infection with larval Echinococcus multilocularis was recognized in one of eight Norway rats, Rattus norvegicus, caught indoors in 2009 in Ebetsu, Hokkaido, northern Japan. Cystic lesions were found in the right median and lateral lobes of the liver, with numerous alveolar cysts in the periphery of the lesions. Protoscolices were formed within large cysts. The laminated layers of the cy...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2002
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.70.7.3969-3972.2002